Experiment Chromatography Of Food Dyes
This experiment is to determine the presence of a mixture in a substance. Chromatography is used to separate substance in a mixture or separating components from a mixture. Using chromatography with a solvent we are able to separate the different mixtures found in a substance. Chromatography can tell if a sample is pure or if it is made up of several different substances.
Experiment & Observation
I gathered together my items; distilled water, salt, ruler, scissors, stapler pencil, tape, 16 toothpicks, Kool-Aid drink mix strawberry and grape, set of McCormick food coloring red, yellow, green and blue, small bag of M&M candy, plastic beaker 50 mL, petri dish 60mm, well-plate 24, FDC blue dye #1 0.5 mL vial, FDC blue dye #2 0.5 mL vial FDC red dye #3 0.5 mL vial, FDC red dye #40 0.5 mL vial, FDC yellow dye #5 0.5 mL vial, FDC yellow dye #6 0.5 mL vial, unknown 0.5 mL vial, 3 filter paper chrom 14x7 cm.
I first made a solvent using a 50 mL beaker with warm room temperature distilled water and a pinch of salt. I made sure the salt was completely dissolved and set the solvent aside.
I took out two of the Chromatography filter paper sheets 14x7 and with a pencil very lightly drew a horizontal line 1 cm from the bottom of both sheet. Then horizontally on the line I just drew I marked off 9 spaces 1.5 cm apart. On one sheet inside the spaces I marked B1, then B2 in the next space and so on for the FD&C food color dyes, at the end I marked KS and KG for Kool-Aid strawberry and grape. On the second sheet of Chromatography filter pater I did the same thing. I made a 1 cm line from the bottom and spaced it off in 1.5 cm spaces. I marked those with the Store Food Colors, the M&M colors and the unknown color. I then opened the FDC dye colors that came in the labpaq and placed three drops of each dye into one well of the 24 well-plate. I took a toothpick and dipped it into the first well and briefly...