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Title: Identification Different Blood Cells and Separation of Red Blood Cells from Other Types Blood Cells. Essay

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SURNAME: THOKOANE
NAMES: BOKAMOSO L.
STUDENT No: 201006580
GROUP: S
COURSE CODE: BIO211
INSTITUTION: UNIVERSITY OF BOTSWANA
EXERCISE 1: INVISTIGATING BLOOD CELLS
TITLLE: IDENTIFICATION DIFFERENT BLOOD CELLS AND SEPARATION OF RED BLOOD CELLS FROM OTHER TYPES BLOOD CELLS.

DATE: 30/08/2011

TITLE: IDENTIFICATION DIFFERENT BLOOD CELLS AND SEPARATION OF RED BLOOD CELLS FROM OTHER TYPES BLOOD CELLS.
AIM: The purpose this study is to identify different cell types from blood and to separate red blood cells from other cells of the blood by centrifugation.

INTRODUCTION
Sadava e.t al (2010) States that Blood is a connective tissue. It consists of cells suspended in an extracellular matrix of complex, yet specific composition. The unusual feature of blood is that the extracellular matrix is a liquid, so blood is a fluid tissue. According to Standring e.t al (2008) Blood is an opaque fluid with a viscosity great than that of water. It is bright red when oxygenated, in the systemic arteries and dark red to purple when deoxygenated, in systemic veins. Blood is a mixture of a clear liquid, plasma and cellular elements. The functions of blood are in metabolism and its regulation, transport, osmotic balance and in the body defense mechanism in the immune system. Russell e.t al (1982).
In this experiment different components of blood was studied under a light microscope after a blood smear was stained with Wright-Giemsa Stain, phosphate buffer and then washed off with distilled water. According to Russell e.t al (1982) the use of polychrome methylene blue and Oesin Y which are now used in the Wright-Giemsa Stain solution were developed by Giemsa Romanowsky in 1891. He observed that this combination of dyes gave excellent selective staining of blood films. After staining the blood the Erythrocytes should appear yellowish-red to tawny,Polymorphonuclear neutrophils’ cytoplasm should appear pale
Pink to tan, the nuclei, reddish-purple. The Eosinophils’ granules should appear...

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